A simple, rapid and accurate reverse phase-high performance liquid chromatographic method for the simultaneous determination of lamivudine
نویسندگان
چکیده
column (250×4.6 mm, 5 μm) in isocratic mode, the mobile phase consisted of methanol, acetonitrile and 0.05 M phosphate buffer (adjusted to pH 4.5 with ortho-phosphoric acid) at a ratio of 60:20:20 v/v/v, and a flow rate of 1.0 mL/min. The eluents were monitored at 254 nm. The retention time of lamivudine and efavirenz were found to be 2.50 min and 4.25 min, respectively. The linear ranges were found to be 10-60
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